Chapter category: Agricultural Biotechnology

Development of Biosensors for the Detection of Hydrogen Peroxide

This chapter appears in the following book:

Biotechnological Applications of Photosynthetic Proteins: Biochips, Biosensors and Biodevices

Edited by: Maria Teresa Giardi and Elena V. Piletska
ISBN: 0-387-33009-7
» Get more information about this book at landesbioscience.com «

Chapter authors:
Louisa Giannoudi, Elena V. Piletska and Sergey A. Piletsky

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Biosensors are a term used for a number of devices either used to monitor living systems or incorporating biotic elements. In this work, the principal applications in the history of their development are reviewed primarily for their use as sensors for detection of analytes such as hydrogen peroxide (H2O2). The important areas of H2O2 application include industrial (pharmaceutical, food, clinical) and environmental analyses. Its use as an antibacterial agent added to milk, and the environmental need to avoid halogenated substances for disinfection purposes, makes H2O2 an important substance in the food and beverage industry. This has raised extensive demands for establishing protocols for H2O2 detection depending on its application. Additionally, it is one of the most important product or substrate of enzyme catalysed oxidation reactions. Besides being a product/substrate of enzymatic reaction, hydrogen peroxide is by itself an important analyte. It plays an important role in natural oxidation processes as it is found in air, solids and water. For the application of PS-II, hydrogen peroxide has being reported in the past to act as an electron donor. This process is through the two-electron oxygen reduction to H2O2 by various synthetic quinones that can react directly to the reducing part of photosystem II. In the case of higher plants, this would require treatment of the thylakoids in the chloroplast cell in order to achieve a hydrogen peroxide production and recognition.

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Chapter category: Agricultural Biotechnology

Development of Biosensors for the Detection of Hydrogen Peroxide

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