Chapter category: Heat Shock Proteins
Preconditioning of Cardiac Myocytes: Studies Using Cultured Neonatal
Heat Shock Proteins in Myocardial Protection
Edited by: Rakesh C. KukrejaISBN: 1-58706-021-3
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Chapter authors:
Jan A. Post, Chris T.W.M. Schneijdenberg and Arie J. Verkleij
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The acquisition of tolerance towards myocardial ischemia and reperfusion can be acquired by several preconditioning procedures. The original preconditioning protocol described for myocardial tissue was reported by Murry et al1 and comprised a few short periods of ischemia of 5 to 10 minutes. Since induction of cross-tolerance between various stresses has been known for quite some time, attempts were made to induce tolerance towards ischemia by preconditioning the hearts or animals by elevated temperatures. These studies showed both a protective effect and the absence of a protective effect.2-5 In order to be able to study specific responses of the myocardial myocytes during preconditioning and/or ischemia/reperfusion and to discriminate between responses of muscle and non-muscle cells in the intact heart, a very valuable tool is a system in which isolated myocardial cells can be subjected to conditions which mimic ischemia and/or reperfusion. The last ten years we have been working on and with a system of simulated ischemia and reperfusion. This model of simulated ischemia elicits responses of the myocytes, which closely resemble the in vivo and in vitro responses of the myocytes in situ in the heart. In this Chapter we would like to discuss the model we developed, the insight it gave in possible mechanisms resulting in myocyte injury during ischemia/reperfusion and the of use this system to study preconditioning in cultured neonatal rat heart cells at a functional level and at gene-expression level.
With regard to preconditioning it is concluded that Hsp70 cannot be the exclusive factor responsible for providing tolerance towards ischemia. Furthermore, it is shown that a preceding heat shock can even result in increased cellular damage during a subsequent ischemic insult, depending on the height of the heat shock temperature and the length of the recovery phase.
Additional chapters from this book:
Ischemia/Reperfusion Injury and Heat Shock Proteins
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Physiological Role of Heat Shock Protein 27
Dipak K. Das and Nilanjana Maulik
Heat shock protein 27 (Hsp27) is a stress–inducible cytosolic protein that is ubiquitously present in many normal cells. The synthesis of Hsp27 is induced by heat shock and other envir...
Stress Proteins in Myocardial Protection: Culture Shock Protein, Heme Oxygenase–1 (Hsp32), Induced by Sublethal Stresses Protects the Heart Against Oxidative Stress
Shiro Hoshida
The formation of a stress protein comprises a mechanism of cell protection highly conserved in evolution. As the induction of stress proteins makes cells more tolerant towards a second, mor...
Antisense, Heat Shock Proteins and the Heart
A. A. Knowlton
Antisense technology provides a tool with which to dissect the components of the stress response. There are two known endogenous sets of protective proteins, the heat shock proteins (Hsps) and...
Preconditioning of Cardiac Myocytes: Studies Using Cultured Neonatal
Jan A. Post, Chris T.W.M. Schneijdenberg and Arie J. Verkleij
The acquisition of tolerance towards myocardial ischemia and reperfusion can be acquired by several preconditioning procedures. The original preconditioning protocol described for myocardia...
Hsp72 in the Regulation of TNF–a Production:
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Myocardial ischemia/reperfusion induces the production of the cardiac depressant cytokine tumor necrosis factor–a (TNF–a). Macrophages (M
Hsp70 and Ischemia Tolerance in the Compromised Heart Ger J. van der Vusse
Luc H.E.H. Snoeckx, Richard N.M. Cornelussen, Robert S. Reneman and
The discovery in eukaryotic and prokaryotic organisms of a prompt and specific response to heat shock, known as the heat shock response, has stimulated the development of a complete new res...
Role of Heat Shock Proteins, Protein Kinase C and ATP-Sensitive Potassium Channel in Delayed Myocardial Protection
Rakesh C. Kukreja, Yong-Zhen Qian and Jeffery B. Hoag
Heat shock proteins (Hsp's) are one of the highly conserved proteins in existence, found in every organism.1 These proteins are synthesized quickly and intensely in response to stre...
Cardioprotection by Stress Proteins
Heat Shock Proteins in Myocardial Protection, edited by Rakesh C. Kukreja
Myocardial ischemia causes a series of changes at the intracellular level within the cardiomyocyte. These intracellular changes include changes in calcium levels, altered osmotic control, memb...
Heat Stress Proteins: A Possible Route to Myocardial Protection
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Heat stress proteins (hsp) are induced by a variety of stimuli including elevated temperature,1 ischemia,2 hypoxia,3 pressure overload4 and some ...
Stress Proteins in Myocardial Protection
Richard Carroll and Derek M. Yellon
In all organisms examined heat stress results in the synthesis of a specific group of proteins known as the heat shock or stress proteins (Hsps). Cells that accumulate these proteins adapt ...
