Chapter category: Drug Design
Red Blood Cell Loading
Erythrocyte Engineering for Drug Delivery and Targeting
Edited by: Mauro MagnaniISBN: 0-306-47691-6
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Chapter authors:
Luigia Rossi, Sonja Serafini and Mauro Magnani
In this Chapter a selection of procedures for the encapsulation of a wide range of molecules into red blood cells is reported. Electroporation, drug-induced endocytosis, osmotic pulsing and hypotonic hemolysis are described. Among the hypotonic hemolysis procedures, three different methods (dilutional, preswell dilutional and dialysis) are described. A critical comparison of several parameters suggests that the dialysis method, expecially that based on a high hematocrit dialysis procedure, is the preferred one since it permits one to obtain both a good percentage of drug incorporation and a good red cell recovery. However, when a procedure moves from the laboratory to the clinic, the availability of appropriate equipment becomes very important. Finally, a new procedure and a new apparatus for the encapsulation of drugs for human therapy, based on two sequential hypotonic dilutions followed by concentration with hemofilter, is described.
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